Journal: PLoS ONE

Article Title: A High Throughput In Vivo Assay for Taste Quality and Palatability

doi: 10.1371/journal.pone.0072391

Figure Lengend Snippet: Potencies and Efficacies of Sweeteners.

Article Snippet: Rebaudioside A, stevioside, luo han guo, and SC45647 were acquired from Redpoint Bio Corp (Ewing, NJ).

Techniques:

Journal: PLoS ONE

Article Title: A High Throughput In Vivo Assay for Taste Quality and Palatability

doi: 10.1371/journal.pone.0072391

Figure Lengend Snippet: Eight sweeteners (SUC = sucrose, REB A = rebaudioside A, ACE K = acesulfame potassium, SCR = sucralose, SAC = saccharine, STEV = stevioside, GLY = L-glycine, and SC45647) were tested for both taste quality and palatability across a range of concentration. Positive control: 100 mM SUC = sucrose; Negative controls: 10 mM CIT = citrate, 1 mM QUI = quinine, water and 100 mM NaCl. Upper panels shows graphs of sweet taste quality plotted as percent of responses made on the sucrose lever. Lower panel shows concentration–response functions for palatability, plotted as mean licks/trial, obtained in the same experiment. Each concentration of test article was dispensed into a single well of a 96-well plate. Data are plotted as mean of responses to contents from a single well per concentration of test article and 6-7 wells for controls per rat, averaged across 4 rats. Error bars are SEM. Data are representative of 3 equivalent experiments. See Figure S5 for plate configuration corresponding to the experiment illustrated in .

Article Snippet: Rebaudioside A, stevioside, luo han guo, and SC45647 were acquired from Redpoint Bio Corp (Ewing, NJ).

Techniques: Concentration Assay, Positive Control

Journal: PLoS ONE

Article Title: A High Throughput In Vivo Assay for Taste Quality and Palatability

doi: 10.1371/journal.pone.0072391

Figure Lengend Snippet: A panel of 15 sweeteners and polycose was tested by a cohort of 4 rats for detection of sweet taste quality (plotted as percent sucrose-appropriate lever presses, upper panel) and palatability (licks/trial, lower panel). Data for each sweetener (both taste quality and palatability) appear directly above and directly below their corresponding labels. Luo Han = Luo han guo, GLZ = glycyrrhizic acid, Reb A = rebaudioside A). Dashed blue and red lines respectively mark the mean responses to water (negative control) and to 100 mM sucrose (positive control). A single low (light gray bars) and single high concentration (dark gray bars) of each test sweetener was dispensed in two wells, and controls in 6-7 wells each, per plate. Concentration pairs were 0.01 and 0.1 mM (SC45647, stevioside, rebaudioside A), 0.1 and 1 mM (saccharin, Ace-K, sucralose), 1 and 10 mM (aspartame, cyclamate, glycyrrhizic acid) and 10 and 100 mM (L-glycine, glucose, maltose, fructose, trehalose). Luo han guo was tested at 1 and 10 mg/ml and polycose at 1% and 10% solutions. Results were averaged across 4 rats and the data shown in the figure are representative of two equivalent experiments. See Figure S6 for plate configuration corresponding to the experiment illustrated in .

Article Snippet: Rebaudioside A, stevioside, luo han guo, and SC45647 were acquired from Redpoint Bio Corp (Ewing, NJ).

Techniques: Negative Control, Positive Control, Concentration Assay